Immunoassay for Cadmium Detection and Quantification

ObjectiveTo detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy (GFAAS) and inductively coupled plasma atomic emission spectroscopy (ICPAES).MethodsAn indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated.ResultsIC-ELISA showed an IC50 of 45.6 μg/L with a detection limit of 1.95 μg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998.ConclusionIC-ELISA can detect and quantify cadmium residue in environmental or food samples.