کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4196305 | 1278673 | 2008 | 6 صفحه PDF | دانلود رایگان |

ObjectiveTo investigate the immunological effect of PM2.5 on cytokine production in female Wistar rats.MethodsFemale Wistar rats were given 0.3 mg, 0.75 mg, 2 mg, 5 mg of PM2.5 per 0.5 mL saline, respectively. Saline was used as the negative control. TNF-α and IL-6 levels in the branchoalveolar lavage were measured by ELISA, and mRNA expression levels in lung tissue were detected by RT-PCR. Alveolar macrophages were collected for testing phogacytic function.ResultsExposure to PM2.5 stimulated TNF-α production in a dose-dependent manner (P < 0.05), However, no statistically significant difference was found. No time-dependent change in TNF-α and IL-6 production was found. TNF-α and IL-6 mRNA expressions were induced by PM2.5-exposure. The phagocytic rate (PR) was significantly decreased by PM2.5 treatment.ConclusionPM2.5 exposure increases inflammation response of the lung in a dose-dependent manner. Moreover, tissue injury induced by PM2.5 may be related to altered production of cytokines. PM2.5 may impair the phagocytic activity of alveolar macrophages.
Journal: Biomedical and Environmental Sciences - Volume 21, Issue 1, February 2008, Pages 63-68