کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4201301 | 1279399 | 2015 | 5 صفحه PDF | دانلود رایگان |
ObjectiveTo determine the effects of Bushenhuoxue formula (BHF) on interleukin-1 beta (IL-1β), transforming growth factor beta 1 (TGF-β1), discoidin domain receptor 2 (DDR2) and matrix metalloproteinase-1 (MMP-1) levels in a rat model of osteoarthritis (OA).MethodsSprague-Dawley rats were used to establish an OA model and subjected to various treatments over 6 weeks. Rats were treated with BHF, glucosamine sulfate (GS), or starch as a control. Serum levels of IL-1β and MMP-1 and joint fluid levels of IL-1β were determined by means of ELISAs. We used immunohistochemistry to determine DDR2 levels in knee cartilage. Gene expression levels of MMP-1 in joint synovial tissue were assessed using reverse transcription polymerase chain reaction assays.ResultsSerum IL-1β levels were unchanged throughout the study. Levels of IL-1β in joint fluid and MMP-1 in sera from the BHF- and GS-treated groups were significantly reduced. DDR2 levels in knee cartilage were also significantly reduced in the BHF group. Expression of the MMP-1 gene was significantly reduced by BHF treatment.ConclusionBHF might be beneficial in the inhibition and alleviation of local inflammatory responses and cartilage degeneration in OA.
Journal: Journal of Traditional Chinese Medicine - Volume 35, Issue 2, 15 April 2015, Pages 192-196