کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4212542 | 1280703 | 2006 | 10 صفحه PDF | دانلود رایگان |

SummaryBackgroundInhaled endotoxin or lipopolysaccharide (LPS) is implicated in the pathogenesis of pulmonary diseases. We investigated the inhalation effects of two different doses of LPS in healthy human subjects.MethodsEighteen healthy non-atopic human subjects inhaled either 15 μg (n=10)(n=10) or 50 μg (n=8)(n=8)Escherichia coli LPS in an open study. As control, each subject had isotonic saline inhalation 1 week before (baseline) and after LPS inhalation. Data collected included those of clinical parameter, induced sputum and peripheral blood CD4+ and CD8+ T cells.ResultsAcute flu-like symptoms and pyrexia were significantly greater in the 50 μg than 15 μg LPS group. Similarly, the increase in sputum and blood total cell and neutrophil counts at 6 h following inhaled LPS were greater in the 50 μg group. Myeloperoxidase, human neutrophil elastase and interleukin-8 in sputum sol, but not blood, showed a trend towards greater increase following 50 μg LPS. All these changes were resolved at one week. In the 50 μg dose group alone, there was a reduction in the proportion of peripheral blood interferon (IFN)-γ-producing CD4+ and CD8+ T cells at 6 h followed by an increase at 1 week after inhaled LPS.ConclusionsThe airway and systemic effects of inhaled LPS are dose-related and predominantly neutrophilic. The changes in the proportions of circulating CD4+ and CD8+ T cells suggests preferential recruitment of IFN-γ-producing T cells into tissue from inhaled 50 μg LPS, followed by reappearance of these cells in blood 1 week later.
Journal: Respiratory Medicine - Volume 100, Issue 3, March 2006, Pages 519–528