کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4312733 1612988 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evidence for impaired function of dopaminergic system in Wfs1-deficient mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
Evidence for impaired function of dopaminergic system in Wfs1-deficient mice
چکیده انگلیسی

Immunohistological studies suggest abundant expression of Wfs1 protein in neurons and nerve fibers that lie in the vicinity of dopaminergic (DA-ergic) fibers and neurons. Therefore, we sought to characterize the function of DA-ergic system in Wfs1-deficient mice. In wild-type mice, amphetamine, an indirect agonist of DA, caused significant hyperlocomotion and increase in tissue DA levels in the dorsal and ventral striatum. Both effects of amphetamine were significantly blunted in homozygous Wfs1-deficient mice. Motor stimulation caused by apomorphine, a direct DA receptor agonist, was somewhat stronger in Wfs1-deficient mice compared to their wild-type littermates. However, apomorphine caused a similar reduction in levels of DA metabolites (3,4-dihydroxyphenylacetic acid and homovanillic acid) in the dorsal and ventral striatum in all genotypes. Behavioral sensitization to repeated treatment with amphetamine (2.5 mg/kg) was observed in wild-type, but not in Wfs1-deficient mice. The expression of DA transporter gene (Dat) mRNA was significantly lower in the midbrain of male and female homozygous mice compared to wild-type littermates. Altogether, the blunted effects of amphetamine and the reduced gene expression of DA transporter are probably indicative of an impaired functioning of the DA-ergic system in Wfs1-deficient mice.


► Amphetamine-induced hyperlocomotion was blunted in Wfs1-deficient mice.
► Amphetamine-induced elevation of dopamine levels in the ventral and dorsal striatum was inhibited in Wfs1-deficient mice.
► These mice did not develop behavioral sensitization to amphetamine.
► Dopamine transporter gene levels were significantly lower in Wfs1-deficient mice.
► Dopamine D2 receptor gene in the ventral striatum remained unchanged after Wfs1 gene disruption.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Behavioural Brain Research - Volume 244, 1 May 2013, Pages 90–99
نویسندگان
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