Shuttle box learning in zebrafish (Danio rerio)

Zebrafish is used in forward genetic and drug screening and is gaining popularity in behavioral brain research but high throughput learning paradigms are lacking. The sight of conspecifics has been shown to be rewarding in zebrafish. Here, in a novel paradigm, subjects learn to respond to alternating presentation of computer-animated zebrafish images. The simplicity and computerization of the paradigm will make it useful for high throughput screening.