Temporal parameters of one-trial tolerance to benzodiazepines in four-plate test–retest

Anxiolytic-like effect of diazepam is abolished by a previous exposure to four-plate test (FPT). Variations of temporal parameters: interval between trials and duration of Trial 1, with or without electric punishments allow characterizing factors which are responsible for this loss phenomenon. Complete spatial representation of FPT seems to be responsible of this one-trial tolerance, and needs at least a 30 s exposure to the apparatus to be completed, with or without punishments.