کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4319863 1290835 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sea urchin embryonic development provides a model for evaluating therapies against β-amyloid toxicity
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Sea urchin embryonic development provides a model for evaluating therapies against β-amyloid toxicity
چکیده انگلیسی

Accumulation of β-amyloid protein is an Alzheimer's disease hallmark but also may be mechanistically involved in neurodegeneration. One of its cleavage peptides, Aβ42, has been used to evaluate the mechanisms underlying amyloid-induced cytotoxicity and targeting of acetylcholine systems. We studied Sphaerechinus granularis sea urchin embryos which utilize acetylcholine and other neurotransmitters as morphogens. At a threshold concentration of 0.1 μM Aβ42, there was damage to the larval skeleton, accumulation of ectodermal cells in the blastocoele and underdevelopment of larval arms. Raising the Aβ42 concentration to 0.2–0.4 μM produced anomalies depending on the stage at which Aβ42 was introduced: at the first cleavage divisions, abnormalities appeared within 1–2 cell cycles; at the mid-blastula stage, the peak period of sensitivity to Aβ42, gastrulation was blocked; at later stages, there was progressive damage to the larval skeleton, digestive tract and larval spicules, as well as regression of larval arms. Each of these anomalies could be offset by the addition of lipid-permeable analogs of acetylcholine (arachidonoyl dimethylaminoethanol), serotonin (arachidonoyl serotonin) and cannabinoids (arachidonoyl vanillylamine), with the greatest activity exhibited by the acetylcholine analog. These results indicate that sea urchin embryos provide a model suitable to characterize the mechanisms underlying the cytotoxicity of Aβ42, as well as providing a system that enables the rapid screening of potential therapeutic interventions. The protection provided by neurotransmitter analogs, especially that for acetylcholine, points to unsuspected advantages of existing therapies that enhance cholinergic function, as well as indicating novel approaches that may prove protective in Alzheimer's disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 75, Issue 1, 31 January 2008, Pages 94–100
نویسندگان
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