Diphenyl diselenide and 2,3-dimercaptopropanol increase the PTZ-induced chemical seizure and mortality in mice

The aim of the present study was to evaluate the interaction between a classic GABAergic antagonist — pentylenetetrazol (PTZ) with an organoselenium compound — diphenyl diselenide (PhSe)2 and with the metal chelating agent — 2,3 dimercaptopropanol (BAL). Mice were pre-treated with 150 μmol/kg (PhSe)2 or BAL (250, 500 or 1000 μmol/kg) before treatment with PTZ. Pre-treatment with (PhSe)2 reduced the latency for PTZ-induced seizure at doses of 40 and 60 mg/kg and cause a decrease in the latency for PTZ-induced death at the dose of 60 mg/kg. However, treatment with PTZ at dose of 80 mg/kg was not affected by (PhSe)2 pre-treatment. Pre-treatment with BAL reduced the latency for PTZ-induced seizure at doses of 40 and 50 mg/kg. In addition, the latency for PTZ-induced death at the dose of 40 mg/kg was decreased significantly by pre-treatment with all doses of BAL. At the dose of 50 mg/kg, a significant decrease in the latency for death occurred only in mice pre-treated with 500 and 1000 μmol/kg of BAL. Our results indicate that the PTZ-induced chemical seizures and mortality was enhanced by (PhSe)2 and BAL. These results indicated that (PhSe)2 and BAL interact with PTZ possibly by modulating the GABAergic system.