کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4320841 | 1291540 | 2015 | 13 صفحه PDF | دانلود رایگان |
• A light-sensitive mu-opioid-like receptor (opto-MOR) was generated and characterized
• Opto-MOR initiates canonical MOPR signaling both in vitro and in neurons
• Photoactivation of opto-MOR in selected GABAergic neurons induces reward or aversion
• Opto-MOR is a novel tool for in vivo optodynamic control of opioid signaling
SummaryOptogenetics is now a widely accepted tool for spatiotemporal manipulation of neuronal activity. However, a majority of optogenetic approaches use binary on/off control schemes. Here, we extend the optogenetic toolset by developing a neuromodulatory approach using a rationale-based design to generate a Gi-coupled, optically sensitive, mu-opioid-like receptor, which we term opto-MOR. We demonstrate that opto-MOR engages canonical mu-opioid signaling through inhibition of adenylyl cyclase, activation of MAPK and G protein-gated inward rectifying potassium (GIRK) channels and internalizes with kinetics similar to that of the mu-opioid receptor. To assess in vivo utility, we expressed a Cre-dependent viral opto-MOR in RMTg/VTA GABAergic neurons, which led to a real-time place preference. In contrast, expression of opto-MOR in GABAergic neurons of the ventral pallidum hedonic cold spot led to real-time place aversion. This tool has generalizable application for spatiotemporal control of opioid signaling and, furthermore, can be used broadly for mimicking endogenous neuronal inhibition pathways.
Journal: - Volume 86, Issue 4, 20 May 2015, Pages 923–935