Cux2-Positive Radial Glial Cells Generate Diverse Subtypes of Neocortical Projection Neurons and Macroglia

• Individual E10.5 Cux2+ RGCs generate both deep and upper layer neurons and glia
• Cux2+ RGCs generate diverse subtypes of molecularly defined projection neurons
• Neurons generated by E10.5 Cux2+ RGCs project subcerebral axons

SummaryWe recently published genetic lineage-tracing experiments using the Fezf2 and Cux2 loci. These experiments demonstrated that at both the clonal and population levels Fezf2+ RGCs are multipotent and that at the population level Cux2+ RGCs are multipotent. Here, we extend our work on the lineages of Fezf2+ and Cux2+ RGCs. Clonal analysis of E10.5 neocortical progenitors suggests that most, if not all, Cux2+ and Fezf2+ RGCs generate diverse projection neuron subtypes located throughout layers 2–6. These results support our previous conclusion that both Fezf2+ and Cux2+ RGCs are multipotent neocortical progenitors. This Matters Arising Response paper addresses the Gil-Sanz et al. (2015) Matters Arising paper, published concurrently in Neuron.