Descending Control of Swim Posture by a Midbrain Nucleus in Zebrafish

• A midbrain nucleus with spinal projections is involved in diverse motor behaviors
• Optogenetic activation evokes smooth steering of the tail via an identified muscle
• Ablation of the nucleus impairs postural control during visually evoked swimming
• These data support a fine-grained modularity of motor control in vertebrates

SummaryThe reticular formation in the brainstem controls motor output via axonal projections to the hindbrain and spinal cord. It remains unclear how individual groups of brainstem neurons contribute to specific motor functions. Here, we investigate the behavioral role of the nucleus of the medial longitudinal fasciculus (nMLF), a small group of reticulospinal neurons in the zebrafish midbrain. Calcium imaging revealed that nMLF activity is correlated with bouts of swimming. Optogenetic stimulation of neurons in the left or right nMLF activates the posterior hypaxial muscle and produces a graded ipsilateral tail deflection. Unilateral ablation of a subset of nMLF cells biases the tail position to the intact side during visually evoked swims, while sparing other locomotor maneuvers. We conclude that activity in the nMLF provides postural control of tail orientation and thus steers the direction of swimming. Our studies provide an example of fine-grained modularity of descending motor control in vertebrates.