کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4321799 1291652 2012 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-Resolution Proteomics Unravel Architecture and Molecular Diversity of Native AMPA Receptor Complexes
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
High-Resolution Proteomics Unravel Architecture and Molecular Diversity of Native AMPA Receptor Complexes
چکیده انگلیسی

SummaryAMPA-type glutamate receptors (AMPARs) are responsible for a variety of processes in the mammalian brain including fast excitatory neurotransmission, postsynaptic plasticity, or synapse development. Here, with comprehensive and quantitative proteomic analyses, we demonstrate that native AMPARs are macromolecular complexes with a large molecular diversity. This diversity results from coassembly of the known AMPAR subunits, pore-forming GluA and three types of auxiliary proteins, with 21 additional constituents, mostly secreted proteins or transmembrane proteins of different classes. Their integration at distinct abundance and stability establishes the heteromultimeric architecture of native AMPAR complexes: a defined core with a variable periphery resulting in an apparent molecular mass between 0.6 and 1 MDa. The additional constituents change the gating properties of AMPARs and provide links to the protein dynamics fundamental for the complex role of AMPARs in formation and operation of glutamatergic synapses.


► AMPARs are multiprotein assemblies with unappreciated complexity and diversity
► AMPARs are assembled from a pool of 34 proteins, 21 are newly identified constituents
► Coassembly of known and novel subunits generates AMPARs with diverse properties
► The proteome of AMPARs provides fundamental links to their complex cell physiology

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 74, Issue 4, 24 May 2012, Pages 621–633
نویسندگان
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