کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4321896 1291660 2009 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Role for SNAP25 in Internalization of Kainate Receptors and Synaptic Plasticity
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
A Role for SNAP25 in Internalization of Kainate Receptors and Synaptic Plasticity
چکیده انگلیسی

SummaryRegulation of surface insertion and internalization of AMPA and NMDA receptors has emerged as a key mechanism for the control of synaptic strength. Regulatory elements for synaptic kainate receptors (KARs) are, however, largely undetermined. We have found that SNAP25 is critical for the synaptic removal of KARs, acting via GluK5 (i.e., KA2) subunits. SNAP25 coimmunoprecipitates with protein complexes containing PICK1, GRIP1, and GluK5 and colocalizes with GluK5 in both hippocampal neurons and transfected HEK293 cells. In hippocampal slices, purified SNAP25 antibodies and blocking peptides caused a GluK5-dependent run-up of KARs-mediated EPSC (EPSCKAR) recorded from CA3 pyramidal neurons when included in the patch pipette and prevented activity-dependent long-term depression of EPSCKAR. As EPSCKAR LTD, SNAP25/PICK1/GluK5 interactions are dynamically regulated by PKC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 63, Issue 3, 13 August 2009, Pages 357–371
نویسندگان
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