کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4322887 1291738 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CLC-3 Channels Modulate Excitatory Synaptic Transmission in Hippocampal Neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
CLC-3 Channels Modulate Excitatory Synaptic Transmission in Hippocampal Neurons
چکیده انگلیسی

SummaryIt is well established that ligand-gated chloride flux across the plasma membrane modulates neuronal excitability. We find that a voltage-dependent Cl− conductance increases neuronal excitability in immature rodents as well, enhancing the time course of NMDA receptor-mediated miniature excitatory postsynaptic potentials (mEPSPs). This Cl− conductance is activated by CaMKII, is electrophysiologically identical to the CaMKII-activated CLC-3 conductance in nonneuronal cells, and is absent in clc-3−/− mice. Systematically decreasing [Cl−]i to mimic postnatal [Cl−]i regulation progressively decreases the amplitude and decay time constant of spontaneous mEPSPs. This Cl−-dependent change in synaptic strength is absent in clc-3−/− mice. Using surface biotinylation, immunohistochemistry, electron microscopy, and coimmunoprecipitation studies, we find that CLC-3 channels are localized on the plasma membrane, at postsynaptic sites, and in association with NMDA receptors. This is the first demonstration that a voltage-dependent chloride conductance modulates neuronal excitability. By increasing postsynaptic potentials in a Cl− dependent fashion, CLC-3 channels regulate neuronal excitability postsynaptically in immature neurons.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 52, Issue 2, 19 October 2006, Pages 321–333
نویسندگان
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