کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4322917 | 1291742 | 2006 | 12 صفحه PDF | دانلود رایگان |
SummaryEarly in C. elegans development, signaling between bilaterally symmetric AWC olfactory neurons causes them to express different odorant receptor genes. AWC left-right asymmetry is stochastic: in each animal, either the left or the right neuron randomly becomes AWCON, and the other neuron becomes AWCOFF. Here we show that the nsy-4 gene coordinates the lateral signaling that diversifies AWCON and AWCOFF neurons. nsy-4 mutants generate 2 AWCOFF neurons, as expected if communication between the AWC neurons is lost, whereas overexpression of nsy-4 results in 2 AWCON neurons. nsy-4 encodes a transmembrane protein related to the γ subunits of voltage-activated calcium channels and the claudin superfamily; it interacts genetically with calcium channels and antagonizes a calcium-to-MAP kinase cascade in the neuron that becomes AWCON. Genetic mosaic analysis indicates that nsy-4 functions both cell-autonomously and nonautonomously in signaling between AWC neurons, providing evidence for lateral signaling and feedback that coordinate asymmetric receptor choice.
Journal: - Volume 51, Issue 3, 3 August 2006, Pages 291–302