کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4322948 1291744 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RGS Expression Rate-Limits Recovery of Rod Photoresponses
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
RGS Expression Rate-Limits Recovery of Rod Photoresponses
چکیده انگلیسی

SummarySignaling through G protein-coupled receptors (GPCRs) underlies many cellular processes, yet it is not known which molecules determine the duration of signaling in intact cells. Two candidates are G protein-coupled receptor kinases (GRKs) and Regulators of G protein signaling (RGSs), deactivation enzymes for GPCRs and G proteins, respectively. Here we investigate whether GRK or RGS governs the overall rate of recovery of the light response in mammalian rod photoreceptors, a model system for studying GPCR signaling. We show that overexpression of rhodopsin kinase (GRK1) increases phosphorylation of the GPCR rhodopsin but has no effect on photoresponse recovery. In contrast, overexpression of the photoreceptor RGS complex (RGS9-1·Gβ5L·R9AP) dramatically accelerates response recovery. Our results show that G protein deactivation is normally at least 2.5 times slower than rhodopsin deactivation, resolving a long-standing controversy concerning the mechanism underlying the recovery of rod visual transduction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 51, Issue 4, 17 August 2006, Pages 409–416
نویسندگان
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