کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4322967 1291745 2007 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hair Bundles Are Specialized for ATP Delivery via Creatine Kinase
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Hair Bundles Are Specialized for ATP Delivery via Creatine Kinase
چکیده انگلیسی

SummaryWhen stimulated strongly, a hair cell's mechanically sensitive hair bundle may consume ATP too rapidly for replenishment by diffusion. To provide a broad view of the bundle's protein complement, including those proteins participating in energy metabolism, we used shotgun mass spectrometry methods to identify proteins of purified chicken vestibular bundles. In addition to cytoskeletal proteins, proteins involved in Ca2+ regulation, and stress-response proteins, many of the most abundant bundle proteins that were identified by mass spectrometry were involved in ATP synthesis. After β-actin, the cytosolic brain isoform of creatine kinase was the next most abundant bundle protein; at ∼0.5 mM, creatine kinase is capable of maintaining high ATP levels despite 1 mM/s ATP consumption by the plasma-membrane Ca2+-ATPase. Consistent with this critical role in hair bundle function, the creatine kinase circuit is essential for high-sensitivity hearing as demonstrated by hearing loss in creatine kinase knockout mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 53, Issue 3, 1 February 2007, Pages 371–386
نویسندگان
, , , , , , , , , ,