کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4323462 1291776 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Single Vesicular Glutamate Transporter Is Sufficient to Fill a Synaptic Vesicle
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
A Single Vesicular Glutamate Transporter Is Sufficient to Fill a Synaptic Vesicle
چکیده انگلیسی

SummaryQuantal size is the postsynaptic response to the release of a single synaptic vesicle and is determined in part by the amount of transmitter within that vesicle. At glutamatergic synapses, the vesicular glutamate transporter (VGLUT) fills vesicles with glutamate. While elevated VGLUT expression increases quantal size, the minimum number of transporters required to fill a vesicle is unknown. In Drosophila DVGLUT mutants, reduced transporter levels lead to a dose-dependent reduction in the frequency of spontaneous quantal release with no change in quantal size. Quantal frequency is not limited by vesicle number or impaired exocytosis. This suggests that a single functional unit of transporter is both necessary and sufficient to fill a vesicle to completion and that vesicles without DVGLUT are empty. Consistent with the presence of empty vesicles, at dvglut mutant synapses synaptic vesicles are smaller, suggesting that vesicle filling and/or transporter level is an important determinant of vesicle size.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 49, Issue 1, 5 January 2006, Pages 11–16
نویسندگان
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