کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4323724 1613807 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chronic low-dose melatonin treatment maintains nigrostriatal integrity in an intrastriatal rotenone model of Parkinson’s disease
ترجمه فارسی عنوان
درمان ملاطون مزمن با دوز کم، یکپارچگی نیتروستریاتیت را در یک مدل رتینون درمورد بیماری پارکینسون حفظ می کند
کلمات کلیدی
بیماری پارکینسون، ملاتونین با دوز کم، محافظت از عصب هیدروکسیلاز تیروزین، روتنون، اینترستریاتال
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


• Rotenone causes behavioral and dopaminergic changes in the nigrostriatal pathway.
• Melatonin normalizes behavior and striatal TH immunoreactivity in lesioned animals.
• Melatonin prevents the loss of TH+ dopamine neurons in the substantia nigra.

Parkinson׳s disease is a major neurodegenerative disorder which primarily involves the loss of dopaminergic neurons in the substantia nigra and related projections in the striatum. The pesticide/neurotoxin, rotenone, has been shown to cause systemic inhibition of mitochondrial complex I activity in nigral dopaminergic neurons, with consequent degeneration of the nigrostriatal pathway, as observed in Parkinson׳s disease. A novel intrastriatal rotenone model of Parkinson׳s disease was used to examine the neuroprotective effects of chronic low-dose treatment with the antioxidant indoleamine, melatonin, which can upregulate neurotrophic factors and other protective proteins in the brain. Sham or lesioned rats were treated with either vehicle (0.04% ethanol in drinking water) or melatonin at a dose of 4 µg/mL in drinking water. The right striatum was lesioned by stereotactic injection of rotenone at three sites (4 μg/site) along its rostrocaudal axis. Apomorphine administration to lesioned animals resulted in a significant (p<0.001) increase in ipsilateral rotations, which was suppressed by melatonin. Nine weeks post-surgery, animals were sacrificed by transcardial perfusion. Subsequent immunohistochemical examination revealed a decrease in tyrosine hydroxylase immunoreactivity within the striatum and substantia nigra of rotenone-lesioned animals. Melatonin treatment attenuated the decrease in tyrosine hydroxylase in the striatum and abolished it in the substantia nigra. Stereological cell counts indicated a significant (p<0.05) decrease in dopamine neurons in the substantia nigra of rotenone-lesioned animals, which was confirmed by Nissl staining. Importantly, chronic melatonin treatment blocked the loss of dopamine neurons in rotenone-lesioned animals. These findings strongly support the therapeutic potential of long-term and low-dose melatonin treatment in Parkinson׳s disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1633, 15 February 2016, Pages 115–125
نویسندگان
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