Ultrastructure of spines and associated terminals on brainstem neurons controlling auditory input

• We examined spines of tensor tympani and stapedius motoneurons and olivocochlear neurons.
• The spines are associated with synaptic terminals that engulf the spine.
• Morphometry of synaptic vesicles demonstrates three types of terminals on each group of neuron.
• Spine terminals on motoneurons contain pleomorphic vesicles, suggesting inhibition.
• Spine terminals on olivocochlear neurons contain round vesicles, suggesting excitation.

Spines are unique cellular appendages that isolate synaptic input to neurons and play a role in synaptic plasticity. Using the electron microscope, we studied spines and their associated synaptic terminals on three groups of brainstem neurons: tensor tympani motoneurons, stapedius motoneurons, and medial olivocochlear neurons, all of which exert reflexive control of processes in the auditory periphery. These spines are generally simple in shape; they are infrequent and found on the somata as well as the dendrites. Spines do not differ in volume among the three groups of neurons. In all cases, the spines are associated with a synaptic terminal that engulfs the spine rather than abuts its head. The positions of the synapses are variable, and some are found at a distance from the spine, suggesting that the isolation of synaptic input is of diminished importance for these spines. Each group of neurons receives three common types of synaptic terminals. The type of terminal associated with spines of the motoneurons contains pleomorphic vesicles, whereas the type associated with spines of olivocochlear neurons contains large round vesicles. Thus, spine-associated terminals in the motoneurons appear to be associated with inhibitory processes but in olivocochlear neurons they are associated with excitatory processes.