کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4324853 1613942 2013 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced dendritogenesis and axogenesis in hippocampal neuroblasts of LRRK2 knockout mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Enhanced dendritogenesis and axogenesis in hippocampal neuroblasts of LRRK2 knockout mice
چکیده انگلیسی

Adult neurogenesis, the formation of new neurons in the mammalian forebrain, is one important mechanism maintaining lifelong neuronal plasticity. The generation and maturation of adult neural stem and progenitor cells is impaired in models of neurodegenerative diseases, in particular Parkinson's disease (PD). Monogenetic forms of PD were identified and associated with several genes including the leucine-rich-repeat kinase 2 (LRRK2). Some of the underlying mechanisms in neurodegenerative diseases are closely linked to neuronal plasticity, and induce changes in adult neurogenesis, neuritic maintenance, synaptic transmission, and neural connectivity.We investigated adult neurogenesis and neuritic development of newly formed neurons in the hippocampal dentate gyrus of LRRK2 knockout mice. Proliferation and survival of newly generated cells were unchanged. However, the expression profile of maturation markers in surviving newly generated cells was altered. While immature neuronal phenotypes were significantly increased, the mature neuronal phenotype of surviving cells remained unchanged. Importantly, the absolute number of immature doublecortin positive neuroblasts was significantly increased in the hippocampus of LRRK2 knockout mice. These neuroblasts presented extended dendritic length with a more complex arborization. Furthermore, LRRK2 deletion resulted in an increased volume of the axonal mossy fiber bundle projecting from dentate granule cells to CA3 pyramidal neurons.Our findings suggest that LRRK2 influences neurogenesis and particularly neuronal morphogenesis. As neurogenesis and the pre-/post- synaptic compartments are significantly altered in PD, our data advance LRRK2 as a potent candidate in addressing neuroregenerative processes.


► Hippocampal proliferation and survival of new cells are unchanged in LRRK2 KO mice.
► The total number of DCX+ neuroblasts is significantly increased in LRRK2 KO mice.
► Increased immature BrdU/DCX, but not mature BrdU/NeuN neuronal fate in LRRK2 KO mice.
► Dendritic length and arborization of DCX+DG neurons is increased in LRRK2 KO mice.
► The overall mossy fiber volume (SP+IIP MFs) is increased in LRRK2 KO mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1497, 25 February 2013, Pages 85–100
نویسندگان
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