کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4324903 1613946 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabotropic glutamate receptor 5 (mGluR5) regulates proliferation and differentiation of neuronal progenitors in the developmental hippocampus
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Metabotropic glutamate receptor 5 (mGluR5) regulates proliferation and differentiation of neuronal progenitors in the developmental hippocampus
چکیده انگلیسی

Metabotropic glutamate receptor 5 (mGluR5) is involved in neural stem cell self-renewal, proliferation, differentiation and survival. In this study, we aimed to further determine the role of mGluR5 in the development of hippocampus using mGluR5 deficit (mGluR5−/−) and wild type (mGluR5+/+) mice at different developmental ages. We showed that the number of BrdU, NeuroD and DCX immunopositive cells was reduced significantly in mGluR5−/− than in mGluR5+/+ mice from postnatal 7 days (P7) to P28, but not at P60. The length and intensity of DCX immunopositive apical dendrites in the dentate gyrus of mGluR5−/− mice were much shorter and lower than in mGluR5+/+ mice respectively at P14, P21 and P28. NeuN immunostaining indicated an accelerated maturation of hippocampal neurons in mGluR5−/− mice. When mGluR5+/+ mice were treated with 2-methyl-6-(phenylethynyl) pyridine (MPEP), a selective antagonist of mGluR5, decreased proliferation of progenitor cells was observed in the hippocampus at early postnatal developmental stages. At P14, there were more BrdU+ cells in the stratum granulosum and subgranular layer of the dentate gyrus in mGluR5+/+ than in mGluR5−/− mice, but the percentage of BrdU++NeuroD+/BrdU+ in the dentate gyrus did not change significantly between the two genotypes of mice. Western Blot study suggested that programmed neuronal death was p53-dependent apoptosis in the developmental hippocampus in mGluR5+/+ mice.


► mGluR5 in the development of hippocampus was studied in mGluR5+/+ and mGluR5−/− mice.
► BrdU, NeuroD and DCX immunopositive cells were reduced in P7 and P28 mGluR5−/− mice.
► DCX positive apical dendrites in the dentate gyrus was longer in mGluR5+/+ mice.
► mGluR5 antagonist MPEP reduced proliferation of progenitor cells in the hippocampus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1493, 1 February 2013, Pages 1–12
نویسندگان
, , , ,