کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4325590 1614015 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Thrombin-induced autophagy: A potential role in intracerebral hemorrhage
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Thrombin-induced autophagy: A potential role in intracerebral hemorrhage
چکیده انگلیسی

Autophagy occurs in the brain after intracerebral hemorrhage (ICH) and thrombin contributes to ICH-induced brain injury and cell death. In this study, we investigated whether thrombin may activate autophagy (in vivo and in cultured astrocytes) and its potential role in ICH. Autophagy was examined using electron microscopy, conversion of light chain 3(LC3) from the LC3-I form to LC3-II, cathepsin D Western blotting and monodansylcadaverine (MDC) staining to detect autophagic vacuoles. 3-Methyladenine (3-MA) was used as an autophagy inhibitor. In vivo, we found that intracaudate injection of thrombin increased conversion of LC3-I to LC3-II, cathepsin D levels, and formation of autophagic vacuoles in the ipsilateral basal ganglia. ICH-induced upregulation of LC3-I to LC3-II conversion and cathepsin D levels was reduced by a thrombin inhibitor, hirudin. In cultured astrocytes, thrombin enhanced the conversion of LC3-I to LC3-II and increased MDC-labeled autophagic vacuoles. 3-MA inhibited thrombin-induced autophagic vacuole formation and exacerbated thrombin-induced cell death. These results indicate that thrombin activates autophagy in the brain and that thrombin has a role in ICH-induced autophagy.


► Thrombin causes autophagy in brain and cultured astrocytes.
► Hirudin, an inhibitor of thrombin, reduces intracerebral hemorrhage-induced autophagy.
► 3-Methyladenine, an inhibitor of autophagy, attenuates autophagic vacuole accumulation after thrombin exposure.
► 3-Methyladenine aggravates thrombin-induced cell death.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1424, 18 November 2011, Pages 60–66
نویسندگان
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