Perinatal development of melanopsin expression in the mouse retina

The melanopsin-expressing retinal ganglion cells are specialized in measuring irradiance for several functions, including daily photoentrainment and regulation of pupil size. In the present study, these cells were analyzed in mice during their perinatal period, from embryonic day (E) 15 to postnatal day (P) 1. Melanopsin expression was detected at E15 in cells that did not co-express the transcription factor Brn3a. Under light/dark (LD) cycles, the number of melanopsin-expressing cells did not change between E16 and E19, while a very significant increase was observed during the short interval around birth, between E19 (the day before birth) and P0 (the day of birth). As these samples were collected after lights on, to determine whether such increase in melanopsin expression was driven by light, we also analyzed samples collected 0–4 hours after birth (during the night period), which revealed that the cell number increase was already present and, therefore, was not induced by the early post-birth light exposure. To clarify the role of ambient light conditions during this period, P1 retinas from pups under constant light or darkness conditions were also analyzed and compared to those of mice under LD cycles. No variation in the number of immunostained cells was detected among the groups studied, indicating that ambient conditions did not provoke the increase in melanopsin expression detected. Rather, it might have been induced by either a maternal or a developmental signal and is likely related to the first connections between the retina and the suprachiasmatic nucleus reported by other authors.

► Melanopsin expression was studied during development (E15-P1) of the mouse retina.
► No colocalization of melanopsin/Brn3a transcription factor was observed at E15.
► No significant variation in melanopsin cell number was found between E16 and E19.
► Significant increase in melanopsin cell number was detected around birth (E19-P1).
► This ambient-condition-independent change suggests maternal/developmental signalling.