کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4325928 | 1614047 | 2011 | 8 صفحه PDF | دانلود رایگان |
The primary afferent neurons are enriched for F-actin in the peripheries, giving rise to ring-like appearances when stained with fluorescence probes for actin. A variety of data, mainly obtained from in vitro experiments, suggested that the satellite cell (SC) envelopes around sensory neurons may be a candidate for the F-actin positive structures. However, immunohistochemical studies on dorsal root ganglia (DRGs) suggested that F-actin was located exclusively within the cortical regions of the sensory neurons. Given this discrepancy, the present study has reexamined the distribution of F-actin in the DRGs at both the fluorescence light and electron microscopic levels. Double staining of F-actin with S-100beta and neural cell adhesion molecule showed that the fluorescence signals for F-actin around the DRG neurons were restricted to the inner margins of the SC envelope. Subsequent ultrastructural observations, by use of a modified anti-FITC system, demonstrated that the F-actin-positive sites corresponded mostly to the adneuronal SC lamellae, while the cortical cytoskeletons in the neurons seemed to contribute little to the typical F-actin staining. The high concentration of F-actin in the SC envelope has not yet described before, and is thought to assist in understanding the functions of the SCs in the ganglia.
Research highlights
► We examine F-actin in sensory ganglia by a phalloidin-based method.
► F-actin is localized mainly in glial components.
► F-actin is concentrated in the adneuronal or adaxonal region.
► The cortical cytoskeletons in neurons contribute little to the typical staining.
Journal: Brain Research - Volume 1392, 25 May 2011, Pages 54–61