A translationally relevant thromboembolic stroke model for the study of secondary hemorrhage after thrombolysis in rats

► A model reflects secondary hemorrhage after thrombolysis in cerebral ischemia. ► It causes consistent vascular occlusion without spontaneous reperfusion. ► The injection of rt-PA induces good reperfusion in the MCA territory. ► Delayed thrombolysis, artery hypertention, and reperfusion increase secondary hemorrhage.