کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4326729 | 1614091 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Modeling seizure-related behavioral and endocrine phenotypes in adult zebrafish
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Modeling seizure-related behavioral and endocrine phenotypes in adult zebrafish Modeling seizure-related behavioral and endocrine phenotypes in adult zebrafish](/preview/png/4326729.png)
چکیده انگلیسی
Larval zebrafish (Danio rerio) have recently been suggested as a high-throughput experimental model of epilepsy-related pathogenetic states. Here we use adult zebrafish to study behavioral symptoms associated with drug-evoked seizures. Experimental epilepsy-like states were evoked in zebrafish by exposure for 20Â min to three chemoconvulsant drugs: caffeine (250Â mg/L; 1.3Â mM), pentylenetetrazole (1.5Â g/L; 11.0Â mM) and picrotoxin (100Â mg/L; 0.17Â mM). Fish behavior was analyzed using manual and video-tracking methods (Noldus Ethovision XT7). Compared to their respective controls, all three drug-treated groups showed robust seizure-like responses (hyperactivity bouts, spasms, circular and corkscrew swimming) accompanied by elevated whole-body cortisol levels (assessed by ELISA). In contrast, control fish did not display seizure-like behaviors and had significantly lower cortisol levels. Paralleling behavioral and endocrine phenotypes observed in clinical and rodent studies, our data implicates adult zebrafish as an emerging experimental model for epilepsy research.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1348, 12 August 2010, Pages 209-215
Journal: Brain Research - Volume 1348, 12 August 2010, Pages 209-215
نویسندگان
Keith Wong, Adam Stewart, Thomas Gilder, Nadine Wu, Kevin Frank, Siddharth Gaikwad, Christopher Suciu, John DiLeo, Eli Utterback, Katie Chang, Leah Grossman, Jonathan Cachat, Allan V. Kalueff,