کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4326986 1614105 2010 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Relationships between large neutral amino acid levels in plasma, cerebrospinal fluid, brain microdialysate and brain tissue in the rat
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Relationships between large neutral amino acid levels in plasma, cerebrospinal fluid, brain microdialysate and brain tissue in the rat
چکیده انگلیسی

Experimental limitations may preclude direct measurement of large neutral amino acid (LNAA) levels in brain tissue. Some data suggest that serum or cerebrospinal fluid (CSF) may provide an index of LNAA brain levels. We examined this in a series of experiments in rats, administering tyrosine, phenylalanine or valine IP 60 min prior to harvesting of blood, CSF or brain tissue or during in vivo microdialysis of the brain. Serum indices of the administered LNAA generally showed a significant (r > 0.8) correlation with brain tissue levels but the linear relationships varied significantly across brain regions, the LNAA and its dose. Increases in levels of an administered LNAA were consistently greater in CSF than in brain tissue. In contrast, changes in LNAA levels in brain tissue and in vivo microdialysate were generally comparable. We confirm that changes in serum and CSF LNAA levels can support limited, qualitative inferences about changes in brain tissue LNAA levels; quantitative inferences should not be drawn without prior validation under relevant experimental conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1334, 2 June 2010, Pages 45–57
نویسندگان
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