کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4328533 1614178 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nuclear contrast angiography: A simple method for morphological characterization of cerebral arteries
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Nuclear contrast angiography: A simple method for morphological characterization of cerebral arteries
چکیده انگلیسی
Visualization of the cerebral vascular tree is important in experimental stroke and cerebral vascular malformation research. We describe a simple method, nuclear contrast angiography, that enables simultaneous visualization of the arterial tree and cerebral endothelial cells in rodent brain whole mounts. A mixture of latex and black ink was injected into the arterial system of rodents, resulting in high contrast demarcation of the arterial tree of the brain. This method clearly differentiates arteries from veins. We applied this method to demonstrate that 14 days of unilateral carotid artery occlusion induces increases in the caliber of (1) bilateral anterior communicating arteries, (2) bilateral anterior cerebral arteries, and (3) ipsilateral proximal middle cerebral artery of the circle of Willis. Unlike other methods, this procedure selectively stains endothelial nuclei of arteries. Thus, cerebral endothelial nuclei can be visualized, quantitated, and morphologically characterized at the same time the cortical arterial tree is delineated. This method should be useful in studies of stroke and cerebral arteriogenesis, which require the accurate assessment of both arterial diameters and endothelial cell density.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1261, 19 March 2009, Pages 75-81
نویسندگان
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