کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4328848 1614193 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression and transport of Angiotensin II AT1 receptors in spinal cord, dorsal root ganglia and sciatic nerve of the rat
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Expression and transport of Angiotensin II AT1 receptors in spinal cord, dorsal root ganglia and sciatic nerve of the rat
چکیده انگلیسی

To clarify the role of Angiotensin II in the regulation of peripheral sensory and motor systems, we initiated a study of the expression, localization and transport of Angiotensin II receptor types in the rat sciatic nerve pathway, including L4–L5 spinal cord segments, the corresponding dorsal root ganglia (DRGs) and the sciatic nerve. We used quantitative autoradiography for AT1 and AT2 receptors, and in situ hybridization to detect AT1A, AT1B and AT2 mRNAs. We found substantial expression and discrete localization of Angiotensin II AT1 receptors, with much higher numbers in the grey than in the white matter. A very high AT1 receptor expression was detected in the superficial dorsal horns and in neuronal clusters of the DRGs. Expression of AT1A mRNA was significantly higher than that of AT1B. AT1 receptor binding and AT1A and AT1B mRNAs were especially prominent in ventral horn motor neurons, and in the DRG neuronal cells. Unilateral dorsal rhizotomy significantly reduced AT1 receptor binding in the ipsilateral side of the superficial dorsal horn, indicating that a substantial number of dorsal horn AT1 receptors have their origin in the DRGs. After ligation of the sciatic nerve, there was a high accumulation of AT1 receptors proximal to the ligature, a demonstration of anterograde receptor transport. We found inconsistent levels of AT2 receptor binding and mRNA. Our results suggest multiple roles of Angiotensin II AT1 receptors in the regulation of sensory and motor functions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1246, 30 December 2008, Pages 111–122
نویسندگان
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