کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4329038 | 1614200 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intrastriatal injection of hypoxanthine alters striatal ectonucleotidase activities: A time-dependent effect
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The aim of this study was to investigate the effects of intrastriatal injection of hypoxanthine on ectonucleotidase (E-NTPDases and ecto-5â²-nucleotidase) activities and expressions in the striatum of rats. The effect of pre-treatment with vitamins E and C on the effects elicited by this oxypurine on enzymatic activities and on thiobarbituric reactive substances (TBARS) was also investigated. The effect of pre-incubation with hypoxanthine on nucleotide hydrolysis in striatum homogenate was also determined. Adult Wistar rats were divided into (1) control and (2) hypoxanthine-injected groups. For ectonucleotidase activity determination, the animals were sacrificed at 30Â min, 24Â h and 7Â days after drug infusion. For the evaluation of the expression of NTPDase 1-3 and also ecto-5â²-nucleotidase, TBARS assay and the influence of the pre-treatment with vitamins on ectonucleotidase activities, the animals were sacrificed 24Â h after hypoxanthine infusion. Results show that hypoxanthine infusion significantly inhibited ectonucleotidase activities and increased TBARS only 24Â h after administration. Pre-treatment with vitamins was able to prevent these effects. Moreover, ecto-5â²-nucleotidase expression was increased (80%) at 24Â h after hypoxanthine infusion. We suggest that these hypoxanthine-induced biochemical modifications could, at least in part, participate in the pathophysiology of Lesch Nyhan disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1239, 6 November 2008, Pages 198-206
Journal: Brain Research - Volume 1239, 6 November 2008, Pages 198-206
نویسندگان
Caren S. Bavaresco, Fabria Chiarani, Janaina Kolling, Denise B. Ramos, Giana P. Cognato, Carla D. Bonan, MaurÃcio R. Bogo, João J.F. Sarkis, Carlos A. Netto, Angela T.S. Wyse,