کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4330096 | 1614249 | 2008 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Potassium channels in hippocampal neurones are absent in a transgenic but not in a chemical model of Alzheimer's disease
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کلمات کلیدی
HEPESaCSF4-APβAPP4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulphonic acid4-aminopyridine - 4-آمینوپیریدینAlzheimer's disease - بیماری آلزایمرartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیβ-amyloid protein - پروتئین β-آمیلوئیدβ-amyloid precursor protein - پروتئین پیش ماده β-آمیلوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have investigated using single channel patch-clamp methods potassium channel prevalence in hippocampal neurones from two animal models of AD. Experiments have been carried out on transgenic mice (Tg2576) carrying the Swedish mutation (K670N/M671L) and rats receiving ventricular infusions of okadaic acid. In cell-attached patches from hippocampal neurones from the Tg2576 and control littermate mice there were three principal unitary conductance - 22 pS, 111 pS and 178 pS. The two channels of intermediate and large conductance were voltage-dependent, highly active in cell-attached patches, activity decreasing markedly on hyperpolarisation. The large conductance channel was sensitive to TEA, iberiotoxin, was activated in excised inside-out patches by Ca 2+i and is the type I maxi-K+ channel. Significantly, there was a reduction in the prevalence of a TEA-sensitive 113 pS channel in neurones from TG2576 mice with a corresponding increase in prevalence of the maxi-K+ channel. There was no difference in the characteristics of maxi-K+ between patches in neurones from the transgenic and littermate controls. In the rat model single channel analysis was performed on hippocampal neurons from three groups of animals i.e. non-operated, and these receiving an infusion of vehicle or vehicle with okadaic acid. Three principal unitary conductances of around 18 pS, 118 pS and 185 pS were also observed in cell-attached recordings from these three groups. The intermediate and high conductance channels were blocked by TEA or 4-AP or 140Â mM RbCl. There were no statistically significant differences in the channel prevalence or channel density between the control and test groups.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1190, 23 January 2008, Pages 1-14
Journal: Brain Research - Volume 1190, 23 January 2008, Pages 1-14
نویسندگان
B. Coles, L.A.K. Wilton, M. Good, P.F. Chapman, K.T. Wann,