کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4331176 1614292 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of acetylpuerarin on hippocampal neurons and intracellular free calcium subjected to oxygen–glucose deprivation/reperfusion in primary culture
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Effects of acetylpuerarin on hippocampal neurons and intracellular free calcium subjected to oxygen–glucose deprivation/reperfusion in primary culture
چکیده انگلیسی

Objectives: This study was undertaken to find out the effects of acetylpuerarin on hippocampal neurons and intracellular free calcium in primary culture subjected to oxygen–glucose deprivation/reperfusion. Methods: According to different reperfusion time (1 h, 6 h, 12 h, 24 h), three concentrations (1.6 μmol l− 1, 0.4 μmol l− 1, 0.1 μmol l− 1) of acetylpuerarin, and MK-801 (10 μmol l− 1), a positive control drug, neurons were randomly divided into 21 groups. Each group was observed by inverted phase contrast microscope; neuron viability was measured by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT); intracellular Ca2+ was observed by Fura-2/AM ester through fluorospectrophotometer. Results: The injured neurons were protected and degeneration and necrosis were alleviated in treatment groups of acetylpuerarin and MK-801. Acetylpuerarin increased the neuron viability at high, middle and low concentrations. Fluorescence detection results showed that the calcium concentration in the group treated with acetylpuerarin and MK-801 was lowered in each reperfusion time. Conclusion: Our results demonstrated that acetylpuerarin could protect the hippocampal neurons from ischemia–reperfusion injury in rats by alleviating the morphological damage, increasing neuron viability and decreasing calcium concentration in neuron.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1147, 25 May 2007, Pages 95–104
نویسندگان
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