Carnosine-like immunoreactivity in neurons of the brain of an advanced teleost, the gray mullet (Chelon labrosus, Risso)

The distribution of the dipeptide carnosine (β-alanyl-l-histidine) and the related dipeptides anserine and homocarnosine has been studied by biochemical methods and immunohistochemistry in the brain of mammals and other tetrapods. These studies have indicated the presence of these dipeptides mainly in glial cells and in some neurons (olfactory receptor neurons and certain putative migrating neurons and neuroblasts). Here, we used immunohistochemistry with a polyclonal anti-carnosine antibody and the streptavidin–avidin method to study for the first time the distribution of carnosine/carnosine-related dipeptides in the brain of a teleost fish (the gray mullet Chelon labrosus). In order to assess the neuronal nature of carnosine-immunoreactive cells, we also used double immunofluorescence methods with antibodies to carnosine and to the neuronal protein HuC/D. The results obtained show that carnosine and/or related dipeptides are present in neurons of various brain regions. The carnosine-like-immunoreactive neuronal populations of the optic tectum and cerebellum are described in detail. In the optic tectum, immunoreactivity to carnosine/carnosine-related dipeptides is present in neurons of the stratum album and the stratum griseum centrale. In the cerebellum, immunoreactivity to these dipeptides is localized in Purkinje cells, in putative Golgi and stellate cells, and in many nerve fibers. Carnosine-like immunoreactive cells in mullet brain seem to be specific types of neurons, in line with previous results in a urodele but at variance with previous results in mammals, reptiles and anurans.