Evidence that norepinephrine influences responses to male courtship song and activity within song control regions and the ventromedial nucleus of the hypothalamus in female European starlings

Little is known about the neural regulation of female responses to male courtship displays. Female European starlings use attributes of male song to assess potential mates and can be highly motivated to approach nest boxes broadcasting male song. The catecholamine norepinephrine (NE) regulates attention and arousal and has recently been implicated in motivated/goal-directed behaviors, suggesting a role for NE in female responses to male song. In the present study, treating females with the noradrenergic neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP-4) increased the number of times females were observed on or near a nest box broadcasting sexually motivated male song, suggesting an inhibitory role for norepinephrine in the regulation of female responses to male song. DSP-4 treatment reduced immunolabeling for the immediate early gene ZENK within area X, a region involved in song learning and possibly discrimination, and the ventromedial nucleus of the hypothalamus, a region involved in female sexual behavior. Furthermore, female responses to male song and numbers of ZENK-labeled cells within area X related positively in DSP-4-treated birds but not controls. Female responses to male song and the density of dopamine-β-hydroxylase in area X and another song nucleus LMAN related positively exclusively in DSP-4-treated birds. Similar trends were consistently observed for the ventromedial nucleus of the hypothalamus. These data suggest roles for area X, LMAN, and the ventromedial nucleus of the hypothalamus in female responses to male song and highlight norepinephrine as an important neuromodulator of this behavior.