کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4332572 1292903 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Neuroglobin mRNA expression after transient global brain ischemia and prolonged hypoxia in cell culture
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Neuroglobin mRNA expression after transient global brain ischemia and prolonged hypoxia in cell culture
چکیده انگلیسی

Neuroglobin is a nerve-specific respiratory protein that has been proposed to play an important role in the protection of brain neurons from ischemic and hypoxic injuries. Here, we investigated the regulation of neuroglobin expression after transient global ischemia in the rat brain using mRNA in situ hybridization and under hypoxic stress in cultured neuronal cell lines (PC12, HN33) by quantitative RT-PCR. While neuroglobin mRNA expression was significantly enhanced in cell culture after severe prolonged hypoxia (0–1% O2 for 24 h), we did not find any significant increases in neuroglobin mRNA levels in the rat brain after transient global ischemia. Vegf and Glut1 mRNAs showed increases in the hippocampus as expected. Therefore, it is unlikely that neuroglobin is instrumental in the acute response of neurons to hypoxic or ischemic insults, for which the mammalian brain is not adapted.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1103, Issue 1, 4 August 2006, Pages 173–180
نویسندگان
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