کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4332739 1292909 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of hyperpolarization-activated current (Ih) in dorsal root ganglion neurons innervating rat urinary bladder
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Characterization of hyperpolarization-activated current (Ih) in dorsal root ganglion neurons innervating rat urinary bladder
چکیده انگلیسی

Afferent pathways innervating the urinary bladder consist of myelinated Aδ-fibers and unmyelinated C-fibers. Normal voiding is dependent on mechanoceptive Aδ-fiber bladder afferents that respond to bladder distention. However, the mechanisms for controlling the excitability of Aδ-fiber bladder afferents are not fully understood. We therefore used whole cell patch-clamp techniques to investigate the properties of hyperpolarization-activated, cyclic nucleotide-gated (HCN) currents (Ih) in dorsal root ganglion (DRG) neurons innervating the urinary bladder of rats. The neurons were identified by axonal tracing with a fluorescent dye, Fast Blue, injected into the bladder wall. Hyperpolarizing voltage step pulses from −40 to −130 mV produced voltage- and time-dependent inward Ih currents in bladder afferent neurons. The amplitude and current density of Ih at a holding potential of −130 mV was significantly larger in medium-sized bladder afferent neurons (diameter: 37.8 ± 0.3 μm), a small portion (19%) of which were sensitive to capsaicin (1 μM), than in uniformly capsaicin-sensitive small-sized (27.6 ± 0.5 μm) bladder neurons. In medium-sized bladder neurons, a selective HCN channel inhibitor, ZD7288, dose-dependently inhibited Ih currents. ZD7288 (10 μM) also increased the time constant of the slow depolarization phase of spike after-hyperpolarization from 91.8 to 233.0 ms. These results indicate that Ih currents are predominantly expressed in medium-sized bladder afferent neurons innervating the bladder and that inhibition of Ih currents delayed recovery from the spike after-hyperpolarization. Thus, it is assumed that Ih currents could control excitability of mechanoceptive Aδ-fiber bladder afferent neurons, which are usually capsaicin-insensitive and larger in size than capsaicin-sensitive C-fiber bladder afferent neurons.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1096, Issue 1, 22 June 2006, Pages 40–52
نویسندگان
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