The selective m1 muscarinic antagonist MT-7 blocks pilocarpine-induced striatal Fos expression

Systemic administration of the nonselective muscarinic agonist pilocarpine induces pronounced striatal Fos expression. Intrastriatal injections of the mamba snake toxin MT-7, a highly selective and irreversible m1 muscarinic antagonist, drastically attenuated this response when given 2, but not 8, days before pilocarpine. In contrast, MT-7 did not alter the response to amphetamine (5 mg/kg). These results suggest that pilocarpine induces Fos expression in the striatum as a result of stimulating m1 muscarinic receptors located within this structure and demonstrate the utility of the MT-7 for in vivo studies of cholinergic function.