کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4334780 1294973 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Probing the transcriptome of neuronal cell types
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Probing the transcriptome of neuronal cell types
چکیده انگلیسی

Distinct neuronal cell types acquire and maintain their identity by expressing different genes. Recently it has become feasible to measure this cell type specific expression by isolating and amplifying mRNA from small populations of fluorescently labeled neurons and probing this mRNA with microarrays. Prior to this, most neuronal gene expression studies used tissue homogenates or randomly selected single cells and were, therefore, not well suited to studying transcriptional differences between cell types. Microarray studies of purified cell types have enabled investigators to identify the transcriptional signatures of, for example, subtypes of pyramidal neurons and interneurons in the neocortex, modulatory dopaminergic and serotonergic neurons, and the striatal neurons that form the so-called ‘direct’ and ‘indirect’ pathways through the basal ganglia. These studies are opening up new approaches to understanding brain circuitry, plasticity and pathology and are refining the concept of the neuronal cell type.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Neurobiology - Volume 16, Issue 5, October 2006, Pages 571–576
نویسندگان
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