کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4334872 1614597 2016 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mass spectrometry imaging of rat brain lipid profile changes over time following traumatic brain injury
ترجمه فارسی عنوان
تصویربرداری از طیف سنجی توزیع چگالی مغز موش صحرایی در طول زمان با توجه به آسیب مغزی شدید تغییر می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


• MSI allows the in situ mapping of molecules directly from tissues.
• Brain images showed changes in most lipids classes 3 days after TBI.
• Signaling lipids such as increase in ceramides increased as early as 1 day after the injury.
• More diffuse lipid changes are observed outside of the injured area.
• Ceramides, diacylglycerols and cholesteryl esters are good biomarkers for TBI.

BackgroundMild traumatic brain injury (TBI) is a common public health issue that may contribute to chronic degenerative disorders. Membrane lipids play a key role in tissue responses to injury, both as cell signals and as components of membrane structure and cell signaling. This study demonstrates the ability of high resolution mass spectrometry imaging (MSI) to assess sequences of responses of lipid species in a rat controlled cortical impact model for concussion.New methodA matrix of implanted silver nanoparticles was implanted superficially in brain sections for matrix-assisted laser desorption (MALDI) imaging of 50 μm diameter microdomains across unfixed cryostat sections of rat brain. Ion-mobility time-of-flight MS was used to analyze and map changes over time in brain lipid composition in a rats after Controlled Cortical Impact (CCI) TBI.ResultsBrain MS images showed changes in sphingolipids near the CCI site, including increased ceramides and decreased sphingomyelins, accompanied by changes in glycerophospholipids and cholesterol derivatives. The kinetics differed for each lipid class; for example ceramides increased as early as 1 day after the injury whereas other lipids changes occurred between 3 and 7 days post injury.Comparison with existing method(s)Silver nanoparticles MALDI matrix is a sensitive new tool for revealing previously undetectable cellular injury response and remodeling in neural, glial and vascular structure of the brain.ConclusionsLipid biochemical and structural changes after TBI could help highlighting molecules that can be used to determine the severity of such injuries as well as to evaluate the efficacy of potential treatments.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 272, 15 October 2016, Pages 19–32
نویسندگان
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