کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4334935 1614639 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Validation of a neuropathology score using quantitative methods to evaluate brain injury in a pig model of hypoxia ischaemia
ترجمه فارسی عنوان
اعتبار سنجی نمره نوروپاتولوژی با استفاده از روش های کمی برای ارزیابی آسیب مغزی در یک مدل خوک ایسکمی هیپوکسی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


• Graded neuropathology score used in a perinatal global hypoxia-ischaemia pig model.
• Healthy neuronal cell density correlated with neuropathology score.
• Both methods detected a difference between treatment (normothermia and hypothermia).
• Our neuropathology score is a valid method to assess regional and global brain injury.

BackgroundNeuropathological examination is the classic outcome measure in experimental studies of newborn brain injury to evaluate novel therapies. We have used a graded neuropathology score in an established global model of perinatal hypoxic-ischaemic (HI) injury. We wished to validate the score using cell counting in our model.New method32 newborn pigs underwent a 45 min global HI insult then maintained at normothermia (NT, rectal temperature, Trectal 38.5 °C) for 72 h or mild total body hypothermia (HT, Trectal 37.0 °C) combined with selective head cooling for 48 h and subsequently maintained at NT for 24 h before brain perfusion fixation. A perinatal pathologist scored haematoxylin and eosin stained 6 μm histological sections for injury in the hippocampus and basal ganglia on a 9-step scale (0.0 = no injury, 4.0 = >75% injury). We counted the number of healthy neurons in the hippocampus CA1 region and putamen using morphological criteria in eight random, non-overlapping fields from representative sections.ResultsHealthy neuronal cell density correlated with neuropathology score in the hippocampus CA1 (r = −0.74) and in the putamen (r = −0.75) and both measures detected a difference between groups. The correlation coefficients were better for the NT compared to the HT group in both the hippocampus (r = −0.87 vs. −0.53) and putamen (r = −0.77 vs. −0.54).Comparison with existing methodWe have validated a histological neuropathological scoring system in our model of perinatal HI by showing correlation between neuronal cell count and estimated injury.ConclusionsOur neuropathology score is a valid method to assess brain injury with good reproducibility and sensitivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 230, 15 June 2014, Pages 30–36
نویسندگان
, , , , ,