Enhancement of neuronal cell adhesion by covalent binding of poly-d-lysine

We have prepared the poly-d-lysine (PDL) bound surfaces for neuron cell culture by covalent binding between the poly-d-Lysine and substrates and investigated neuronal cell adhesion properties and cell growth morphology. The number of neuronal cell and the number of neurite per neuronal cell on PDL bound surfaces was much more than those on PDL coated surfaces and also the neuronal cells on PDL bounded surfaces survived a longer time. On the pattern of covalently bound PDL, neuronal cells and their neurites are confined within the grid line leading to patterned neuronal networks with the long-term survival.

► Surface modification through covalent binding between poly-d-lysine and substrates for neuron cell culture.
► The number of neuronal cell and neurite per neuronal cell on PDL bounded surfaces was much more than those on PDL coated surfaces.
► Neuronal cells and their neurites on the pattern of covalently bound PDL are confined within the grid line leading to patterned neuronal networks.