کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4335948 1295190 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impact of microdialysis probes on vasculature and dopamine in the rat striatum: A combined fluorescence and voltammetric study
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Impact of microdialysis probes on vasculature and dopamine in the rat striatum: A combined fluorescence and voltammetric study
چکیده انگلیسی

Measuring extracellular dopamine in the brain of living animals by means of microdialysis and/or voltammetry is a route towards understanding both normal brain function and pathology. Previous reports, however, suggest that the tissue response to implantation of devices may affect the outcome of the measurements. To address the source of the tissue response and its impact on striatal dopamine systems microdialysis probes were placed in the striatum of anesthetized rats. Images obtained by dual-label fluorescence microscopy show signs of ischemia and opening of the blood–brain barrier near the probe tracks. Opening of the blood–brain barrier was further examined by determining dialysate concentrations of carbi-DOPA, a drug that normally does not penetrate the brain. Although carbi-DOPA was recovered in brain dialysate, it did not alter dialysate dopamine levels or evoked dopamine release as measured by voltammetry near the probes. Microdialysis probes also significantly diminished the effect of intrastriatal infusion of kynurenate on extracellular dopamine levels as measured by voltammetry near the probes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 174, Issue 2, 30 September 2008, Pages 177–185
نویسندگان
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