کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4336037 | 1295193 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Image analysis of time-lapse movies—A precision control guided approach to correct motion artefacts Image analysis of time-lapse movies—A precision control guided approach to correct motion artefacts](/preview/png/4336037.png)
In long-term time-lapse studies of cell migration, it is often important to distinguish active movement of individual cells from global tissue motion caused, for instance, by morphogenetic changes, or due to artefacts. We have developed a method to define and correct global movements. This is realized by the sequential morphing of image sequences to the initial image based on the position of immobile reference objects. Technically, the approach is implemented in ImageJ, using the plugin UnwarpJ. We describe an efficient way to select parameter settings such as to optimize image correction. To this end, we implemented a strict statistical control that allows to quantify image registration quality. We document this approach using a time-lapse sequence of migrating interneurons in slice cultures of the developing cerebellum.
Journal: Journal of Neuroscience Methods - Volume 172, Issue 1, 15 July 2008, Pages 67–73