کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4336278 1295203 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Long-term transgene expression in mouse neural progenitor cells modified with ϕC31 integrase
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Long-term transgene expression in mouse neural progenitor cells modified with ϕC31 integrase
چکیده انگلیسی

Stem cells can potentially be utilized in combined gene/cell therapies for neural diseases. We examined the ability of the non-viral ϕC31 integrase system to promote stable transgene expression in mouse neural progenitor cells (mNPCs). ϕC31 integrase catalyzes the sequence-specific integration of attB-containing plasmids into pseudo attP sites in mammalian genomes, to produce long-term transgene expression. We achieved gene transfer by co-nucleofection of a plasmid carrying the luciferase marker gene and an attB site and a plasmid expressing integrase in mNPCs that had been generated in a neurosphere preparation. Luciferase expression was quantified in live cells for 8 weeks, revealing persistence of gene expression. Sequence-specific integration at a preferred pseudo attP site in the mouse genome was detected by using PCR. Furthermore, sustained transgene expression was demonstrated in genetically modified NPCs that were cultured in conditions that promoted either growth or differentiation into neurons and astrocytes. Our results demonstrate that the ϕC31 integrase system produces stable transgene expression in adult mNPCs and their progeny and may be useful in strategies for combating neurodegenerative disorders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 173, Issue 2, 30 August 2008, Pages 299–305
نویسندگان
, , , ,