کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4337119 | 1614658 | 2006 | 10 صفحه PDF | دانلود رایگان |
The automated behavioural apparatus, LABORAS™ (Laboratory Animal Behaviour Observation, Registration and Analysis System), has been further validated with respect to the ability of the system to detect behavioural impairments in mice, following various dopaminergic manipulations. Initially data were obtained from mice administered with amphetamine, haloperidol, SCH23390, apomorphine and l-DOPA, with the focus on locomotor and grooming activities. The data recorded by LABORAS™ on administration of these pharmacological tool compounds, is comparable with published findings using standard LMA systems and conventional observer methods. In addition the home cage behaviour of mice administered with the dopaminergic neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) using an acute dosing regimen was also investigated. In LABORAS™, mice subjected to MPTP lesioning showed deficits in spontaneous motor activity at day 6–7 post-MPTP administration, over a 24 h test period, as compared to saline treated controls. The data captured and analysed using LABORAS™, suggests that the automated system is able to detect both pharmacologically and lesion-induced changes in behaviour of mice, reliably and efficiently.
Journal: Journal of Neuroscience Methods - Volume 156, Issues 1–2, 30 September 2006, Pages 218–227