کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4337266 | 1614741 | 2016 | 11 صفحه PDF | دانلود رایگان |
• IV infusion of MSCs in the chronic phase of SCI improved locomotor function.
• Some infused MSCs distributed to the injured spinal cord.
• IV MSC infusion rapidly reduced intravenous dye leakage into the injured spinal cord.
• RECA-1 and PDGFR β positive microvasculature was more prevalent in the MSC-group.
• MSC-group showed greater anatomical repair in the chronic SCI.
Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat models of spinal cord injury (SCI). However, most studies have focused on the acute or subacute phase of SCI. In the present study, MSCs derived from bone marrow of rats were intravenously infused 10 weeks after the induction of a severe contusive SCI. Open field locomotor function was assessed weekly until 20 weeks post-SCI. Motor recovery was greater in the MSC-treated group with rapid improvement beginning in earlier post-infusion times than in the vehicle-treated group. Blood spinal cord barrier (BSCB) integrity was assessed by the intravenous infusion of Evans Blue (EvB) with spectrophotometric quantitation of its leakage into the parenchyma. In MSC-treated rats, BSCB leakage was reduced. Immunohistochemical staining for RECA-1 and PDGFR-β showed increased microvasculature/repair-neovascularization in MSC-treated rats. There was extensive remyelination around the lesion center and increased sprouting of the corticospinal tract and serotonergic fibers after MSC infusion. These results indicate that the systemic infusion of MSCs results in functional improvement that is associated with structural changes in the chronically injured spinal cord including stabilization of the BSCB, axonal sprouting/regeneration and remyelination.
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Journal: Neuroscience - Volume 335, 29 October 2016, Pages 221–231