کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4337462 1614777 2015 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Altered expression of neuroplasticity-related genes in the brain of depressed suicides
ترجمه فارسی عنوان
بیان تغییرات ژن های مرتبط با نوروپلاستی در مغز خودکشی افسرده
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


• GPM6A and GPM6B are transcriptionally downregulated in the hippocampus of depressed suicides.
• Neuroplasticity-related proteins that form complexes with GPM6A were identified.
• mRNA of the components of GPM6A complexes is decreased in the depressed group.
• Coexpression pattern analysis demonstrates coordinated gene expression in the brain.
• In the brain of depressed suicides this coordinated response is disrupted.

Background: Expression of the neuronal membrane glycoprotein M6a (GPM6A), the proteolipid protein (PLP/DM20) family member, is downregulated in the hippocampus of chronically stressed animals. Its neuroplastic function involves a role in neurite formation, filopodium outgrowth and synaptogenesis through an unknown mechanism. Disruptions in neuroplasticity mechanisms have been shown to play a significant part in the etiology of depression. Thus, the current investigation examined whether GPM6A expression is also altered in human depressed brain. Methods: Expression levels and coexpression patterns of GPM6A, GPM6B, and PLP1 (two other members of PLP/DM20 family) as well as of the neuroplasticity-related genes identified to associate with GPM6A were determined using quantitative polymerase chain reaction (qPCR) in postmortem samples from the hippocampus (n = 18) and the prefrontal cortex (PFC) (n = 25) of depressed suicide victims and compared with control subjects (hippocampus n = 18; PFC n = 25). Neuroplasticity-related proteins that form complexes with GPM6A were identified by coimmunoprecipitation technique followed by mass spectrometry. Results: Results indicated transcriptional downregulation of GPM6A and GPM6B in the hippocampus of depressed suicides. The expression level of calcium/calmodulin-dependent protein kinase II alpha (CAMK2A) and coronin1A (CORO1A) was also significantly decreased. Subsequent analysis of coexpression patterns demonstrated coordinated gene expression in the hippocampus and in the PFC indicating that the function of these genes might be coregulated in the human brain. However, in the brain of depressed suicides this coordinated response was disrupted. Conclusions: Disruption of coordinated gene expression as well as abnormalities in GPM6A and GPM6B expression and expression of the components of GPM6A complexes were detected in the brain of depressed suicides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 299, 23 July 2015, Pages 1–17
نویسندگان
, , , , ,