کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4337841 | 1614824 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gustatory solitary tract development: A role for neuropilins
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کلمات کلیدی
PBSPFANeuropilin-2NeuropilinsSemaphorin-3a1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate - 1،1'-dioctadecyl-3،3،3 '، 3'-tetramethylindocarbocyanine perchlorateDiI - DIISolitary tract - دستگاه تناسلیAxon guidance - راهنمای آکسونSemaphorins - سمفورین هاVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Phosphate buffered saline - فسفات بافر شورneuropilin-1 - نوروپیلن-1paraformaldehyde - پارافرمالدهیدGustatory - گوشت خوار
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The rostral nucleus of the solitary tract (rNST) receives orosensory information from taste bud cells in the tongue and palate via cranial nerves VII and IX. These nerves enter the brainstem, form the solitary tract (ST) and synapse with neurons in the rNST, which then relay incoming sensory information to other brain areas to process external gustatory stimuli. Factors that direct or regulate the trajectory of the developing ST are largely unknown. We used 1,1â²-dioctadecyl-3,3,3â²,3â²-tetramethylindocarbocyanine perchlorate (DiI) to identify ST projections originating from cells in the geniculate ganglia of embryonic rats from embryonic day 14 through 18 (E14-E18). After identifying the ST fibers, immunolabeling for and protein expression analysis of the axon guidance molecules neuropilin-1 (Npn-1) and neuropilin-2 (Npn-2) and their binding partners, semaphorin-3A (Sema-3A) and semaphorin-3F (Sema-3F) were performed. The results detail the formation of ST projections into the gustatory brainstem and their relationship to developing rNST neurons. DiI-labeled ST fibers were present in the brainstem as early as E14. Npn-1 was expressed in the ST and in the trigeminal tract at E14, but levels of the protein declined through E18. The expression levels of the binding partner of Npn-1, Sema-3A, increased from E14 to E18. Npn-2 was expressed in the ST and, additionally, in radially oriented, tuft-like structures within the brainstem at E14. Expression levels of Npn-2 also declined through E18, in contrast to the expression levels of its binding partner, Sema-3F, which increased during this time period. For the first time, the time course and particular molecular components involved in development of the ST have been identified. These results indicate that the neuropilin and semaphorin families of axon guidance molecules are potential molecular participants in ST formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 252, 12 November 2013, Pages 35-44
Journal: Neuroscience - Volume 252, 12 November 2013, Pages 35-44
نویسندگان
SARA L. Corson, MIWON Kim, CHARLOTTE M. Mistretta, ROBERT M. Bradley,